Boss, I’ve just finished a risk matrix for the new recombinant protein pipeline—could we walk through the critical nodes and optimize the workflow?

Here’s the high‑risk node list—probability ≥70% and impact ≥8. 1. Gene synthesis step: owner = Dr. Lin, mitigation = double‑check sequence in silico and confirm primer design before order. 2. Cell line transfection: owner = Alex, mitigation = use automated electroporator and run a pilot run for 24 hrs to validate expression. 3. Protein purification: owner = Maya, mitigation = switch to affinity tag with a rapid wash protocol, and set up real‑time HPLC monitor. 4. Stability assay: owner = Raj, mitigation = run accelerated degradation at 37 °C and 4 °C simultaneously, log results daily. 5. Regulatory documentation: owner = Sarah, mitigation = auto‑populate forms from spreadsheet template and schedule weekly review. All steps are linked in the shared sheet with owners, deadlines, and a live status bar. Let me know if you need more detail.

Status bar is wired to auto‑refresh every minute, no lag expected. I’ve added a weekly checkpoint on Monday 9 AM for each owner—agenda, progress, blockers. If any delay pops up, it’ll trigger an alert in the dashboard. Team is on track, and I’ll ping me if something slips.